报告题目：Functionalize aptamers for on-site small-molecule detection

报告时间：2018年8月13日上午10：00开始

报告地点：嘉锡楼413

报 告 人：肖艺教授（美国佛罗里达国际大学化学与生物化学系）

邀请单位：福州大学食品安全与生物分析教育部重点实验室

摘要：On-site small-molecule detection is important for applications such as environmentalmonitoring, food safety, drug screening, and medical diagnostics. Existing on-site assays such as chemical tests suffer from frequent false-positives and false-negatives, while immunoassays are greatly limited by their short shelf-life and the costly and time-consuming process of developing new target-specific antibodies. These problems can be overcome by using aptamers, oligonucleotide-based bio-affinity elements isolated in vitro via Systematic Evolution of Ligands by EXponential enrichment (SELEX). Recently, my lab has achieved numerous successes related to aptamer isolation, engineering and sensor development. Specifically, we have engineered a variety of new aptamers with different functionalities—such as cooperative target binding, dye displacement, and structure-switching—and utilized these functionalized aptamers to develop a host of innovative aptamer-based sensing platforms that employ a variety of modalities to achieve rapid and sensitive detection of small-molecule targets including ATP, cocaine, dehydroisoadrosterone-3-sulfate, synthetic cathinones, and tetrahydrocannabinol in complex biological samples. Based on these successes, we have developed novel SELEX methods to isolate aptamers with inherent functionalities using rationally designed libraries, selection conditions, and strategies. Such aptamers are customized for specific sensing applications, and therefore represent the most optimal solution for solving real-world problems. All findings reported here have served as a solid foundation for developing novel, customized SELEX methods to generate functionalized aptamers, enabling downstream development of sensors for any small molecule of interest.

个人简介：

肖艺，美国佛罗里达国际大学化学与生物化学系副教授，福州大学化学学院闽江讲座教授。2000年于南京大学获得博士学位（师从陈洪渊院士和鞠熀先教授），博士期间首次利用纳米金的电化学性质设计新型酶传感器。2001年11月至2004年9月，赴以色列耶路撒冷希伯来大学 Itamar Willner 教授课题组从事博士后研究。首次利用纳米金作为电子转移介质构建葡萄糖电化学生物传感器，利用DNAzyme催化检测DNA和利用纳米金尺寸变化快速监测酶反应。2004年10月开始，在美国加州大学圣巴巴拉分校Prof. Alan Heeger 和 Prof. Kevin Plaxco课题组从事博士后研究，利用生物核酸材料设计、发展新型非标记电化学生物传感器。2007年10月至2011年7月，在美国加州大学圣巴巴拉分校 Prof. Tom Soh实验室任助理研究员，期间她在新型适配体筛选技术、DNA和小分子芯片检测等生物技术关键领域取得了突破性进展。2011年8月开始，任美国佛罗里达国际大学化学与生物化学系Associate Professor。主要利用核酸适配体和纳米材料发展小分子药物快速、现场检测方法，以及发展新型功能化适配体筛选技术，并将该技术用于小分子药物和癌症标志物的特异性识别核酸适配体筛选。

近年来在权威期刊上共发表了论文70多篇学术论文，包括1篇Science，18篇JACS，5篇Angew. Chem., Int. Ed.，6篇PNAS，2篇Nano Letters，2篇Nucleic Acids Research等。论文被他引8000多次，H-index > 45。